Immunohistochemistry:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
Labeling:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
Magnetic Cell Separation:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
Western Blot:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
Staining:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
Immunofluorescence:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
FACS:Article Title: Human Bone‐Derived Endothelial Cells Mediate Bone Regeneration via Distinct Expression of KIT Ligand
Article Snippet: .. Antibody Vendor Cat number Dilution* Mouse anti-human Vimentin (V9) Abcam Ab8069 1:200 (IF) Rabbit anti-Osterix (OSX) Abcam ab22552 1:200 (IF) Goat anti-human/mouse CD117/c-Kit Novus Biologicals AF1356 1:50 (IF, IHC) Mouse anti-human CD31 (JC70A, human specific) Agilent M082329-2 1:200 (IF) Mouse anti-human CD34 Antibody BioLegend 343608 1:200 (IF) Rabbit anti-human Osteocalcin (OCN) Proteintech 23418-1-AP 1:200 (IF) Rat anti-human RUNX2/CBFA1 (232902) R&D Systems MAB2006 1:200 (IF) Rabbit anti-human Von Willebrand Factor (vWF) DAKO A0082 1:200 (IF) Mouse anti-human Osteocalcin (OCN) R&D Systems MAB1419-SP 1:200 (IF) Rabbit anti-α smooth muscle actin (1A4) Sigma-Aldrich A2547 1:300 (IF) Rhodamine labeled Ulex Europaeus Agglutinin I (UEA I, human specific) Vector Laboratories RL-1062 1:100 (IF) PE anti-human CD90 (Thy1) BioLegend 328109 1:100 (MACS) Texas Red -conjugated horse anti-mouse IgG Vector Laboratories TI-2000 1:200 (IF) FITC-conjugated horse antimouse IgG Vector Laboratories FI-2000 1:200 (IF) Peroxidase-conjugated horse anti-mouse IgG Vector Laboratories PI-2000 1:200 (IHC) Texas Red-conjugated goat anti-rabbit IgG Vector Laboratories TI-1000 1:200 (IF) FITC-conjugated goat antirabbit IgG Vector Laboratories FI-5000 1:200 (IF) Donkey anti-Rat IgG ThermoFisher A78946 1:200 (IF) Donkey anti-Goat IgG ThermoFisher A32814 1:200 (IF) Notch1 (D1E11) XP® Rabbit mAb Cell Signaling Technology 3608S 1:1000 (WB) Human Osteopontin/OPN Antibody R&D Systems MAB14331-100 1:1000 (WB) Anti-GAPDH antibody Millipore Sigma G9545-100UL 1:1000 (WB) Anti-Rabbit-HRP Kindle Biosciences R1004 1:1000 (WB) Anti-Mouse-HRP Kindle Biosciences R1004 1:1000 (WB) * IHC: immunohistochemistry staining; IF: immunofluorescence staining; MACS: magnetic- activated cell sorting; WB: western blot. ..
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